Protocol for performing angiogenic and tumorigenic assays using the in ovo chick embryo chorioallantoic membrane model
The chick embryo chorioallantoic membrane (CAM) is a viable alternative in vivo model to explore cancer biology due to its simplicity, accessibility, and cost-effectiveness. Here, we present a protocol for performing angiogenic and tumorigenic assays using the in ovo chick embryo CAM model. We describe steps for initial general preparation and for the angiogenic and tumorigenic assays in two distinct sections.
Authors
- Roberto Ronca (ORCID: https://orcid.org/0000-0001-8979-7068)
- Andrei Turtoï (ORCID: https://orcid.org/0000-0003-3813-6635)
- Tommy Chastel
- Stefania Maria Filomena Mitola (ORCID: https://orcid.org/0000-0002-5557-738X)
- Serena Filiberti (ORCID: https://orcid.org/0000-0001-9795-2638)
- Michela Corsini (ORCID: https://orcid.org/0000-0002-8673-4376)
Institutions
- Inserm (FR)
- University of Brescia (IT)
Publication Details
- Journal
- STAR Protocols
- Published
- 2025-02-28
- DOI
- https://doi.org/10.1016/j.xpro.2025.103663
- Citations
- 5
- Primary Topic
- Angiogenesis and VEGF in Cancer
- Type
- article
- Field-Weighted Citation Impact
- 4.03
Funders
- European Commission
- Associazione Italiana per la Ricerca sul Cancro