Biosynthesis of the <i>C</i>‐Glycosylated Depside Arenicolin B
We uncovered the biosynthetic pathway of the C-glycosylated depside arenicolin B from Phialomyces arenicola through genome mining, heterologous reconstitution and biochemical characterization. The biosynthetic gene cluster (BGC) of arenicolin B is comprised of a HRPKS, a NRPKS, and a C-glycosyltransferase. Through biochemical dissection and evaluation, we demonstrate the NRPKS thioesterase (TE) domain catalyses depside formation.
Authors
- Gerald F. Bills (ORCID: https://orcid.org/0000-0003-2352-8417)
- Colin A. Johnson (ORCID: https://orcid.org/0000-0002-2979-8234)
- Bruno Perlatti
- Yi Tang (ORCID: https://orcid.org/0000-0003-1597-0141)
- Lorraine Nuniz
Institutions
- Rutgers, The State University of New Jersey (US)
- University of California, Los Angeles (US)
- Carnegie Department of Plant Biology (US)
- PharmatrophiX (United States) (US)
- Brown Foundation (US)
- The University of Texas Health Science Center at Houston (US)
Publication Details
- Journal
- ChemBioChem
- Published
- 2025-02-28
- DOI
- https://doi.org/10.1002/cbic.202500003
- Citations
- 1
- Primary Topic
- Microbial Natural Products and Biosynthesis
- Type
- article
- Field-Weighted Citation Impact
- 1.39