Antioxidant Potential and Phenolic Content of Plantago major

Objective: This study aimed to evaluate the antioxidant capacity and bioactive compound content of the aerial parts of Plantago major, a medicinal plant with potential therapeutic applications. Methods: The aerial parts were extracted using 70% methanol, and the solvent was evaporated. Total phenolic content (TPC) and total flavonoid content (TFC) were quantified. Antioxidant capacity was assessed using DPPH, ABTS, and FRAP assays. Results: The DPPH radical scavenging assay showed an IC50 value of 127.33 ± 1.07 µg/mL, while the ABTS assay revealed an IC50 value of 46.74 ± 2.53 µg/mL. The FRAP assay demonstrated a reducing power equivalent to 44.62 ± 0.61 mg TE/g extract. Conclusion: The high phenolic and flavonoid content of Plantago major extract contributes significantly to its strong antioxidant activity. These findings suggest its potential application as a natural antioxidant source in the pharmaceutical and nutraceutical industries.