Methods of Possible Application of Cell-Free Amniotic Fluid Analogues in the Practice of a Pediatrician

The use of amniotic fluid as a source of stem cells is of great interest in cosmetology, genetics, obstetrics, neonatology and other areas of medicine. The relatively easy method of cell culture expands and reduces the cost of their use, but their indiscriminate use is fraught with the risk of developing malignant neoplasms due to the lack of control over the differentiation of multipotent cells in the patient’s body. The relatively easy method of cell culture expands and reduces the cost of their use, but their indiscriminate use is fraught with the risk of developing malignant neoplasms due to the lack of control over the differentiation of multipotent cells in the patient’s body. But even cell-free amniotic fluid is rich in a variety of cytokines that promote enterocyte protection against the effects of pro-inflammatory immune response factors and hypoxia, as well as optimal water-electrolyte composition. An analog of cell-free amniotic fluid, which includes tissue growth factors, can be a means of additional protection of the epithelium of the gastrointestinal tract, help inhibit the development of necrotic enterocolitis in reversible stages, which can reduce the number of surgical interventions in newborns and positively affect the healing of damaged epithelium at the site of intestinal wounds. The aim of this study was to find scientific evidence for this hypothesis.