Thermophilic Strain Bacillus subtilis Kb.12.Gl.35 as a Potential Producer of Mannanases for Obtaining Prebiotic Short-Chain Mannan Oligosaccharides

This study aimed to assess the ability of using low-cost plant raw materials to produce mannanases from thermophilic Bacillus strain. The nutrients used for mannanase synthesis by thermophilic strain Bacillus subtilis Kb.12.Gl.35 included amaranth flour and corn extract. Analysis of galactomannan hydrolysates revealed the formation of mannan oligosaccharides (MOS) of various length. MOS promoted the increase of short-chain fatty acids (SCFAs) formation by probiotic Lactobacillus strains. Plant raw materials can be used as a potential substrate for thermostable mannanases production by Bacillus subtilis Kb.12.Gl.35 and for the synthesis of prebiotic oligosaccharides.