Laser Micromechanical Sensor for Measurement of the Mass of Bacterial Cell Colonies
We presented results of applying an adaptive interferometry technique, based on dynamic holograms recorded in photorefractive crystal, to accurately determine the mass of biofilm bacteria colonies by the measuring of microcantilever frequency shift. During the experimental testing, biofilms of Escherichia coli were grown on the surface of a cantilever previously coated with a BaF2 film. The proposed experimental setup is completely non-contact due to using an optical method of excitation and detection of cantilever oscillations. Based on the measured shift in the resonant frequency, the mass of the biofilm grown on the cantilever was calculated to be (2.072 ± 0.003) × 10–9 gram.
Authors
- D. V. Storozhenko (ORCID: https://orcid.org/0000-0002-3521-5269)
- Roman V. Romashko (ORCID: https://orcid.org/0000-0003-0869-0993)
- T. A. Efimov (ORCID: https://orcid.org/0000-0001-5398-9703)
- Konstantin N. Galkin (ORCID: https://orcid.org/0000-0001-5386-1013)
- Anton Degtyarenko
- Igor M. Chernev (ORCID: https://orcid.org/0000-0002-8726-9832)
- Yury Shkryl (ORCID: https://orcid.org/0000-0002-3657-6058)
- M. N. Bezruk (ORCID: https://orcid.org/0000-0003-2127-2462)
Institutions
- Russian Academy of Sciences (RU)
- Institute of Automation and Control Processes (RU)
- Federal Scientific Center of the East Asia Terrestrial Biodiversity FEB RAS (RU)
Publication Details
- Journal
- Bulletin of the Russian Academy of Sciences Physics
- Published
- 2024-12-01
- DOI
- https://doi.org/10.1134/s1062873824709759
- Primary Topic
- Mechanical and Optical Resonators
- Type
- article
- Field-Weighted Citation Impact
- 0.00