Microalgae Spirulina platensis Fortification Enhance Mice Spermatozoa Quality in Combination with Anadara granosa Blood Shell Powder

<b>Background and Objective:</b> Infertility, a condition in reproductive health, refers to the inability to achieve conception after a year of regular unprotected intercourse. This study builds on previous research involving a patented food supplement containing <i>Anadara granosa</i> L. bloodshell powder. The goal is to enhance the quality of blood shell capsules from AnadaraMAN by fortifying them with <i>Spirulina platensis</i>, a highly nutritious microalga. <b>Materials and Methods:</b> Thirty male mice, weighing 20-30 g and aged 8-11 weeks, were divided into 6 treatment groups using the CRD method. The fortification process involved dissolving <i>Anadara granosa</i> L. and <i>Spirulina platensis</i> in a 0.5% Na-CMC solvent according to specific doses, administered orally to the mice twice daily for 21 days. Spermatozoa samples were collected from the cauda epididymis of euthanized mice. Sperm morphology was observed using 1% eosin dye under a 400x microscope, while motility was assessed in a 0.9% physiological NaCl suspension. Quantitative data was then analyzed using the Analysis of Variance (ANOVA) test for normally distributed data and continued with the Least Significant Difference (LSD) test. <b>Results:</b> Findings revealed a safe daily dose for mice is 4.16 mg/20 g of <i>Anadara granosa</i> L. blood shell and 2.6 mg/20 g of <i>Spirulina platensis</i> microalgae. Fortifying significantly increased viscosity in treatment groups Q (50% <i>Anadara granosa</i> L. with 50% <i>Spirulina platensis</i>) and T (100% <i>Anadara granosa</i> L.), as shown by a Kruskal Wallis test with a p-value of 0.002 which is less than the threshold of 0.05. Furthermore, mice spermatozoa displayed normal coloration during examination, suggesting no adverse effects from the supplementation. <b>Conclusion:</b> The study suggests thepotential benefits of combining <i>Anadara granosa</i> L. and <i>Spirulina platensis</i> to enhance reproductive health, highlighting the need for further research.