[Study on the application of immature platelet and thromboelastography in assessing the risk of hemorrhage after blood-poisonous snake bite].

<b>Objective:</b> To explore the value of immature platelets and thromboelastography (TEG) in evaluating bleeding tendency in patients bitten by blood venomous snakes. <b>Methods:</b> In December 2023, a bleeding scoring system was used to grade the bleeding score and degree of bleeding in 147 patients with blood venomous snake bites. Immature platelets and TEG were also tested to analyze the correlation between the bleeding degree of patients and indicators such as TEG, immature platelet ratio (IPF), and absolute value of immature platelets (A-IPF). The measurement data were represented by Mean±SD, and ANOVA was used for comparison among groups. Statistical data were analyzed using <i>χ</i>(2) test. Spearman correlation was used to analyze the correlation between bleeding degree and the platelet count (PLT), immature platelet and TEG. Pearson linear correlation was used to analyze the correlation of PLT, immature platelets and TEG. The indexes of immature platelets and TEG were analyzed by receiver operating characteristic curve. <b>Results:</b> The 147 patients were graded for bleeding severity based on their bleeding scores, and divided into mild (22 cases), moderate (119 cases), and severe groups (6 cases), and the clinical data such as age, gender and the time from bite to visit were compared among the three groups. The differences were not statistically significant (<i>P</i>>0.05). The coagulation reaction time (R), clot formation time (K), and immature platelet parameters (IPF, A-IPF) in TEG parameters increased with the degree of bleeding. PLT decreased with the degree of bleeding, and the difference was statistically significant (<i>P</i><0.05). Blood venomous snake bite patients have a negative correlation between PLT and IPF (<i>r</i>=-0.301, <i>P</i>=0.012), but no correlation with A-IPF (<i>r</i>=0.072, <i>P</i>=0.461) ; PLT was positively correlated with coagulation Angle (αAngle) and maximum thrombus amplitude (MA) of TEG (<i>r</i>=0.220、0.243, <i>P</i>=0.028、0.015), but negatively correlated with R value and K value (<i>r</i>=-0.368、-0.362, <i>P</i><0.001). IPF is positively correlated with R and K values (<i>r</i>=303、271, <i>P</i>=0.0020、0.035) ; IPF is not correlated with MA value and Angle angle, while A-IPF is positively correlated with R value (<i>r</i>=0.162, <i>P</i><0.001). The degree of bleeding in patients is positively correlated with R value (<i>r</i>=0.237, <i>P</i><0.001), but not with K value, MA value, and Angle angle (<i>P</i>>0.05) ; The degree of bleeding in patients is correlated with PLT (<i>r</i>=-0.411, <i>P</i><0.001). The ROC curve analysis results show that the R value, K value, and MA value are effective in evaluating the bleeding risk of blood venomous snake bites in patients, and the PLT combined with A-IPF has the best efficacy in evaluating the bleeding risk of blood venomous snake bites in patients. <b>Conclusion:</b> The combination of PLT and A-IPF, TEG's R value, K value, MA value indicators can accurately evaluate the bleeding risk of blood venomous snake bites, and can be widely used in clinical practice as an important prediction method for bleeding tendency in patients bitten by blood venomous snakes.