Miniaturization of MALDI Mass Spectrometers with the Technological Breakthrough of the Digital Ion Trap: Peptide and Protein Analysis in MS<sup>1</sup>, MS<sup>2,</sup> and MS<sup>3</sup>

A digital ion trap (DIT) mass spectrometer was developed to extend the mass range in comparison to conventional ion traps. This was achieved by changing the RF voltage from a sinusoidal to a rectangular waveform. In addition to the extended mass range, the size of the instrument was miniaturized. To show the benefits of this development, MALDI applications in MS1, MS2, and MS3 are presented: On one hand, it is possible to analyze intact proteins, on the other hand the instrument enables insights into the structure of antibodies and glycoproteins after enzymatic digestion and collision-induced dissociation (CID).