Characterization of the phenolic profile, antioxidant and neuroprotective activity of leaf hydroalcoholic extracts of <i>Euphorbia bupleuroides</i> subsp. <i>luteola</i> (Kralik) maire growing wild in Algeria

The objective of this research was to investigate the phenolic profile and some biological properties of the leaves of <i>Euphorbia bupleuroides</i> subsp. <i>luteola</i> grown wild in Algeria, still unexplored. The leaves were subjected to extraction with 80% MeOH using simple maceration (SM) and ultrasound-assisted maceration (UM) to establish the most efficient conditions for the recovery of phenolic compounds. The study of antioxidant potential suggested that SM and UM extracts displayed a superimposable activity both in the DPPH test and in the reducing power assay. On the other hand, in the Fe2+ chelating activity assay, SM was more active than UM. SM extract prevented damage induced by β-amyloid protein (Aβ) being more effective against ROS generated after treatment with Aβ. In addition, SM exhibited later protection against malondialdehyde. In conclusion, it is possible to state that simple maceration represents an effective technique for the recovery of compounds with antioxidant and neuroprotective properties from <i>E. bupleuroides</i> subsp. <i>luteola</i> leaves.