The Drosophila epidermal growth factor receptor pathway regulates Hedgehog signalling and cytoneme behaviour

During Drosophila epithelial development, dynamic signalling filopodia (cytonemes) establish direct contacts between distant cells to facilitate the formation of the Hedgehog signalling gradient. However, not much is known about how cytonemes are regulated. In this study, we show that cytoneme dynamics and Hedgehog signalling in the Drosophila epithelia depend on the Epidermal Growth Factor pathway and on its downstream effector Ras1. We describe that EGFR/Ras1 pathway is required to maintain in the wing disc epithelium the basal plasma membrane levels of Interference Hedgehog (Ihog), a critical Hh co-receptor and adhesion protein. In addition, our data demonstrate that filamin A or Cheerio in Drosophila, responds to both Ihog and EGFR pathway and recruited to the basal site of the plasma membrane. This recruitment contributes to Ihog's role in stabilizing cytonemes.

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Publication Details

Journal
Nature Communications
Published
2025-02-26
DOI
https://doi.org/10.1038/s41467-025-57162-5
Citations
2
Primary Topic
Hedgehog Signaling Pathway Studies
Type
article
Field-Weighted Citation Impact
1.61

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article

The Drosophila epidermal growth factor receptor pathway regulates Hedgehog signalling and cytoneme behaviour

Ana‐Citlali Gradilla, Isabel Guerrero, James G. Wakefield, D. Sánchez‐Hernández et al.
2 citations
Nature Communications
Hedgehog Signaling Pathway Studies
1.61
article

The Drosophila epidermal growth factor receptor pathway regulates Hedgehog signalling and cytoneme behaviour

Ana‐Citlali Gradilla, Isabel Guerrero, James G. Wakefield, D. Sánchez‐Hernández, Vasiliki Lalioti, Adrián Aguirre‐Tamaral, Sheila Jordán-Álvarez, Carlos Jiménez‐Jiménez, Clara Fernández-Pardo, Irene Sánchez-Platero
article en
2 citations

Abstract

During Drosophila epithelial development, dynamic signalling filopodia (cytonemes) establish direct contacts between distant cells to facilitate the formation of the Hedgehog signalling gradient. However, not much is known about how cytonemes are regulated. In this study, we show that cytoneme dynamics and Hedgehog signalling in the Drosophila epithelia depend on the Epidermal Growth Factor pathway and on its downstream effector Ras1. We describe that EGFR/Ras1 pathway is required to maintain in the wing disc epithelium the basal plasma membrane levels of Interference Hedgehog (Ihog), a critical Hh co-receptor and adhesion protein. In addition, our data demonstrate that filamin A or Cheerio in Drosophila, responds to both Ihog and EGFR pathway and recruited to the basal site of the plasma membrane. This recruitment contributes to Ihog's role in stabilizing cytonemes.

Nature CommunicationsVol. 16(1)
Consejo Superior de Investigaciones Científicas (ES), University of Graz (AT), University of Exeter (GB), Centro de Biología Molecular Severo Ochoa (ES), Universidad Autónoma de Madrid (ES)
Biotechnology and Biological Sciences Research Council
Zero hunger
Openalex Percentile: Top 19%
Hedgehog Signaling Pathway Studies
1.61
DOIOpen Access PDFOpenAlex
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