Investigating the Genetic Association of Selected Candidate Loci with Alopecia Areata Susceptibility in Jordanian Patients

<i>Background and Objectives</i>: Alopecia areata (AA) is a common cell-mediated autoimmune disease of the hair follicle that results in hair loss patches, affecting males and females of all ages and ethnicities. Although its etiology is not fully understood, AA is hypothesized to have a multifactorial basis with a strong genetic association. This study aims to replicate the genetic association of several risk loci in the Jordanian population for the first time. <i>Materials and Methods</i>: Genomic DNA samples of 152 patients with AA and 150 control individuals were extracted from EDTA blood tubes collected from dermatology clinics, in addition to the clinical data of participants. Genetic sequencing of the 21 targeted risk loci was carried out using the Sequenom MassARRAY^® system (iPLEX GOLD), and the results were statistically analyzed using the Statistical Package for the Social Sciences. <i>Results</i>: The results compared the distribution of alleles and genotypes and the association between control individuals and AA patients. However, our results do not support a significant association of all of the 21 SNPs in our AA cohort (<i>p</i> > 0.05). <i>Conclusions:</i> Our data emphasize that AA has a varied genetic component between ethnic groups and suggest that other additional environmental and psychological triggers may be involved.