Evaluation of Moore and grab sampling method for Salmonella Typhi detection in environmental samples in Ghana
Generally the proportion of S.Typhi detections in wastewater samples was less than blood culture-based detections. Limited detection of confirmed typhoid fever cases at the local hospital may reflect healthcare seeking behaviours, access as well as early treatment with over-the-counter antibiotics. Further work is required to confirm these qPCR detections with amplicon sequencing methods. Strategies also needs to be developed for integration of ES into public health decision making for the prevention of typhoid fever.
Authors
- Michael Owusu (ORCID: https://orcid.org/0000-0001-5066-150X)
- Nicolette A. Zhou (ORCID: https://orcid.org/0000-0002-3854-376X)
- Christopher B. Uzzell (ORCID: https://orcid.org/0000-0002-4204-3485)
- Alex Shaw (ORCID: https://orcid.org/0000-0002-3166-872X)
- Nicholas C. Grassly (ORCID: https://orcid.org/0000-0001-6067-4507)
- Sampson Twumasi-Ankrah (ORCID: https://orcid.org/0000-0002-9183-1883)
- Jonathan Rigby (ORCID: https://orcid.org/0000-0001-5143-0981)
- Debora Akortia
- Eric Darko (ORCID: https://orcid.org/0000-0002-7074-115X)
- Yaw Adu‐Sarkodie (ORCID: https://orcid.org/0000-0003-2093-1534)
- John Scott Meschke (ORCID: https://orcid.org/0000-0002-8983-9042)
- Catherine Troman (ORCID: https://orcid.org/0000-0001-8584-7785)
- Michael Owusu-Ansah (ORCID: https://orcid.org/0000-0003-3631-0564)
- Ellis Owusu‐Dabo (ORCID: https://orcid.org/0000-0003-4232-4292)
- Gifty Nkrumah
Institutions
- University of Washington (US)
- Kwame Nkrumah University of Science and Technology (GH)
- Imperial College London (GB)
- Seattle University (US)
Publication Details
- Journal
- PLoS ONE
- Published
- 2025-02-26
- DOI
- https://doi.org/10.1371/journal.pone.0318840
- Citations
- 3
- Primary Topic
- Salmonella and Campylobacter epidemiology
- Type
- article
- Field-Weighted Citation Impact
- 3.49
Funders
- Bill and Melinda Gates Foundation