Transformation of flg22 perception into electrical signals decoded in vasculature leads to sieve tube blockage and pathogen resistance
This study focuses on the question how and where information acquired by FLS2 perception of flg22 is transformed into electrical signals crucial for generation of local and systemic defense responses. In <i>Arabidopsis thaliana</i> and <i>Vicia faba</i> leaves, FLS2 density was high in the epidermis and vascular parenchyma, low in mesophyll, and absent in sieve elements (SEs). Aequorin-based examinations disclosed dual cytosolic Ca<sup>2+</sup> peaks shortly after flg22 application, which corresponded with two voltage shifts from the epidermis to SEs. These signals were converted into rapid long-range action potentials (APs) or slower short-range variation potentials (VPs). Modified phytohormone-levels demonstrated systemic AP effects. Jasmonic acid up-regulation was significantly higher in wild-type than <i>Atseor1/2</i> mutants. Abundant Ca<sup>2+</sup> influx associated with VPs was responsible for transient sieve element occlusion (SEO) near the flg22 perception site, whereas SEO was absent in <i>Atseor1/2</i> and <i>Atfls2</i> mutants. Biological relevance of SEO was demonstrated by higher susceptibility of <i>Atseor1/2</i> mutants to <i>Pseudomonas syringae</i> than wild-type plants.
Authors
- Alexandra C. U. Furch (ORCID: https://orcid.org/0000-0002-9968-753X)
- Gundula A. Noll (ORCID: https://orcid.org/0000-0002-1137-9891)
- Katrin Ehlers (ORCID: https://orcid.org/0000-0001-7110-1595)
- Judith Fliegmann (ORCID: https://orcid.org/0000-0003-1021-8006)
- Edgar Peiter (ORCID: https://orcid.org/0000-0002-9104-3238)
- Veit Grabe (ORCID: https://orcid.org/0000-0002-0736-2771)
- Lisa S. Wrobel
- Matthias R. Zimmermann (ORCID: https://orcid.org/0000-0001-5199-7582)
- Jens B. Hafke
- Dirk Prüfer (ORCID: https://orcid.org/0000-0001-6402-7702)
- Ralf Oelmüller (ORCID: https://orcid.org/0000-0002-3878-0044)
- Sandra S. Scholz (ORCID: https://orcid.org/0000-0002-5389-5296)
- Axel Mithöfer (ORCID: https://orcid.org/0000-0001-5229-6913)
- Aart J. E. van Bel (ORCID: https://orcid.org/0000-0002-1878-5147)
- Karl‐Heinz Kogel (ORCID: https://orcid.org/0000-0003-1226-003X)
- Alexandra Koch
- Tom Haufschild (ORCID: https://orcid.org/0000-0002-4212-674X)
- Jan‐Peter Maaß
- Jonas Nötzold
- Florian Teutemacher
- Stefanie V. Buxa-Kleeberg
Institutions
- Centre National de la Recherche Scientifique (FR)
- Justus-Liebig-Universität Gießen (DE)
- University of Münster (DE)
- Institut de Biologie Moléculaire des Plantes (FR)
- Max Planck Institute for Chemical Ecology (DE)
- Fraunhofer Institute for Molecular Biology and Applied Ecology (DE)
- RheinMain University of Applied Sciences (DE)
- University of Regensburg (DE)
- Université de Strasbourg (FR)
- Martin Luther University Halle-Wittenberg (DE)
- Friedrich Schiller University Jena (DE)
- University of Tübingen (DE)
Publication Details
- Journal
- Science Advances
- Published
- 2025-02-26
- DOI
- https://doi.org/10.1126/sciadv.ads6417
- Citations
- 3
- Primary Topic
- Plant and Biological Electrophysiology Studies
- Type
- article
- Field-Weighted Citation Impact
- 5.25