A Silicon Rhodamine‐fused Glibenclamide to Label and Detect Malaria‐infected Red Blood Cells
The malaria parasite Plasmodium falciparum affects the lives of millions of people worldwide every year. The detection of replicating parasites within human red blood cells is of paramount importance, requiring appropriate diagnostic tools. Herein, we design and apply a silicon rhodamine-fused glibenclamide (SiR-glib). We first test this far-red fluorescent, fluorogenic and endoplasmic reticulum-targeting sulfonylurea in mammalian cells and pancreatic islets, before characterizing its labeling performance in red blood cells infected with the asexual developmental stages of Plasmodium falciparum. We further combine SiR-glib with a portable smartphone-based microscope to easily and rapidly identify parasitized red blood cells, providing proof of principle for diagnostic use in rural endemic areas without major healthcare facilities.
Authors
- Volker Haucke (ORCID: https://orcid.org/0000-0003-3119-6993)
- Johannes Broichhagen (ORCID: https://orcid.org/0000-0003-3084-6595)
- Blaise Gatin‐Fraudet (ORCID: https://orcid.org/0000-0002-9000-9820)
- Dorien A. Roosen (ORCID: https://orcid.org/0000-0002-6261-8151)
- Christiane Huhn (ORCID: https://orcid.org/0000-0001-9862-8784)
- Souvik Ghosh
- Nicole Kilian (ORCID: https://orcid.org/0000-0001-7489-9782)
- Julia Ast (ORCID: https://orcid.org/0000-0002-0039-4762)
- Cindy Close (ORCID: https://orcid.org/0000-0003-4728-033X)
- Philip Tinnefeld (ORCID: https://orcid.org/0000-0003-4290-7770)
- David J. Hodson (ORCID: https://orcid.org/0000-0002-8641-8568)
- Kai Johnsson (ORCID: https://orcid.org/0000-0002-8002-1981)
- Ingo Holtz
- Martin Lehmann (ORCID: https://orcid.org/0000-0002-8370-6353)
- Viktorija Glembockyte (ORCID: https://orcid.org/0000-0003-2531-6506)
- Luc Reymond (ORCID: https://orcid.org/0000-0001-5282-9273)
- Kilian Roßmann (ORCID: https://orcid.org/0000-0003-4965-9669)
- Claudia Bastl
- Mareike Eis
- Michelle Werum
- Smilla Konrad
- Laura Kneller
Institutions
- Delta State University (NG)
- Heidelberg University (DE)
- Center for NanoScience (DE)
- University Hospital Heidelberg (DE)
- California State University, Chico (US)
- Max Planck Institute for Medical Research (DE)
- Leibniz-Forschungsinstitut für Molekulare Pharmakologie (DE)
- The Federal Polytechnic, Ado-Ekiti (NG)
- Oxford Centre for Diabetes, Endocrinology and Metabolism (GB)
- NIHR Surgical Reconstruction and Microbiology Research Centre (GB)
- Forschungsverbund Berlin (DE)
- École Polytechnique Fédérale de Lausanne (CH)
- University of Birmingham (GB)
- Ludwig-Maximilians-Universität München (DE)
Publication Details
- Journal
- ChemBioChem
- Published
- 2025-02-26
- DOI
- https://doi.org/10.1002/cbic.202400628
- Citations
- 1
- Primary Topic
- Malaria Research and Control
- Type
- article
- Field-Weighted Citation Impact
- 1.88
Funders
- Medical Research Council