In Vitro Studies of Neurospheres Stimulated by Nanoelectrodes

This paper reports on the development of nanoelectrodes for electrically stimulating neurospheres. The nanoelectrodes were fabricated using self-assembled microsphere beads and Au coating, integrated within a cell-culture chamber. The viability and differentiation of the cells within the neurospheres under electrical stimulation have been studied for the first time. It has been found that the optimal electrical stimulation voltage is 20 mV for high viability and neuron differentiation of the cells in neurospheres.