Carbon fluxes rewiring in engineered E. coli via reverse tricarboxylic acid cycle pathway under chemolithotrophic condition
This study demonstrates a novel strategy to engineer E. coli for CO<sub>2</sub> fixation using only one or two heterologous enzymes under chemolithotrophic conditions. These findings reveal the minimal genetic and nutritional requirements for CO<sub>2</sub> assimilation and provide insights into metabolic flux partitioning in engineered strains. This research paves the way for sustainable applications in carbon fixation and biotechnological innovation.
Authors
- Tien-Tsai Su
- En‐Pei Isabel Chiang (ORCID: https://orcid.org/0000-0002-0158-0962)
- Chu-Han Huang
- Jian-Hau Peng (ORCID: https://orcid.org/0000-0001-6052-8606)
- Chieh‐Chen Huang (ORCID: https://orcid.org/0000-0002-3739-6315)
- An-I Tsai
- Shou-Chen Lo (ORCID: https://orcid.org/0000-0001-6787-1685)
- Dongyan Wu (ORCID: https://orcid.org/0000-0003-0175-3904)
- Yu-Chieh Chen (ORCID: https://orcid.org/0000-0002-1838-276X)
- Ya‐Tang Yang (ORCID: https://orcid.org/0000-0002-0562-4512)
- Yu-Ning Yu
Institutions
- National Chung Hsing University (TW)
- National Tsing Hua University (TW)
- Academia Sinica (TW)
Publication Details
- Journal
- Journal of Biological Engineering
- Published
- 2025-02-26
- DOI
- https://doi.org/10.1186/s13036-025-00489-w
- Citations
- 9
- Primary Topic
- Microbial Metabolic Engineering and Bioproduction
- Type
- article
- Field-Weighted Citation Impact
- 7.25
Funders
- Ministry of Education