Molecular identification of Zeugodacus cucurbitae (Coquillett, 1899) (Diptera: Tephritidae) using PCR-RFLP

This paper discusses the method of molecular identification of the melon fly Zeugodacus cucurbitae (Coquillett) based on restriction fragment length polymorphismpolymerase chain reaction (PCR-RFLP).This approach, which involves the analysis of nucleotide composition of the 5'-end of the mitochondrial COI gene fragment, allows for the detection of diff erences in the nucleotide sequences of this region and serves as a rapid method for identifi cation and diff erentiation Z. cucurbitae from other fruit fl y species, which larvae can also be found in the fruits of cucurbit plants.. - Zeugodacus cucurbitae (Coquillett) - (-). , 5'- COI, Z. cucurbitae -, .