17 Self-fixing scaffold membrane prevents postoperative adhesion by scavenging ROS production

Abstract Aim This study focuses on the investigation of formation mechanism and prevention technologies for postoperative abdominal adhesions by combining bioengineering technology with the target therapy on abdominal adhesion mechanism. Methods A tissue engineering anti-adhesion membrane (SC-Xg) was prepared by cross-linking xanthan gum (Xg) with sodium citrate (SC) through dehydration condensation reaction for in vivo implantation to prevent postoperative abdominal adhesions. The physicochemical properties of SC-Xg were characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, rheometry, and swelling experiments to screen for suitable material formula ratios. Moreover, the biocompatibility of SC-Xg was assessed by co-culture with cells and fluorescence staining of live/dead cells. Through cellular experiments, we explored the possible mechanisms of sodium citrate inhibition of oxidative stress. Based on in vivo implantation of SC-XG membrane to mouse IBM, the safety and efficacy of SC-Xg in preventing postoperative abdominal adhesions were further confirmed using histopathological staining and ELISA assays. Results We developed a sodium citrate-based cross-linked xanthan gum tissue engineering membrane (SC-Xg) through a dehydration condensation reaction for the prevention of abdominal adhesion formation after abdominal surgery. The mechanical properties of xanthan gum were optimized by using different amount of sodium citrate for cross-linking. The SC-XG membrane showed the antioxidant properties, good self-fixation ability, biocompatibility, and biodegradability, and was easy to prepare for applications. In vitro and in vivo experiments confirmed the clinical translational potential of SC-Xg by shielding the surgical site, inhibiting oxidative stress and inflammatory response by activating Nrf2, and significantly reducing adhesion formation. Conclusions SC-Xg membrance inhibits oxidative stress in the injured peritoneum and prevents the formation of adhesions.