Conventional and Molecular detection of the common etiologic agents of incurable mastitis of goats at various villages of South Egypt

Mastitis is an eminent problem inducing considerable economic detriment to goats in South Egypt. Many mastitic pathogens are incriminated as a major cause of incurable mastitis and Mycoplasma agalactiae is still a missing pathogen. The current work is aimed to detect the mastitis pathogens including Mycoplasma agalactiae in the mammary secretion of goats that did not response to field therapy. A microbial survey on the common pathogens of goats with incurable mastitis was carried-out during three consecutive years starting Jan. 2020. A total number of 890 goats located in 13 villages of four Governorates in southern Egypt were subsequently inspected. The samples were subjected to mycoplasmal and bacteriological examinations. The culturally isolated strains were subjected to PCR technique for molecular identification of Mycoplasma agalactiae. One hundered ninety-five (21.91 %) cases showed signs of clinical mastitis. Multiparous goats were more susceptible than primiparous. Based on the history taken with field veterinarians in Vet. Clinics, 100 (51.28 %) goats were therapeutically irresponsible and encountered as incurable mastitis. Cultural detection with molecular identification revealed that Mycoplasma agalactiae was a prominent pathogen (59.00 %) either alone or joined with others. On the other hand, the second grade was occupied by coagulase negative staphylococci and Staphylococcus aureus by routine bacteriological examinations. These results may designate that Mycoplasma plays an outstanding role in the incurability of goats’ udder affections and the absence of sanitary measures favored a higher prevalence of mastitis in goats. Mycoplasma should tested simultaneously with routine bacteriology of drug resistant caprine mastitis.