Pharmacokinetic and Therapeutic Dose Comparison of Diane-35 and IVF-C Inj 5000 IU in Common Quail

Background and Objective: The use of steroids is regarded as the most argued thorny topic due to mutual positive and negative obtained results. This study examines the pharmaco-histokinetic effects on common quail of two different steroids: IVF-C Inj (containing 5000 IU of human chorionic gonadotropin) and Diane-35 (containing cyproterone acetate-ethinyl estradiol). Materials and Methods: The 120 common quails were split into winter and summer groups for a two-phase in vivo experiment that lasted 10-20 days each. There were 40 treated birds and 20 control birds in each study group, which was then split into two subgroups of 20 birds each. Daily doses of IVF-C Inj (5 IU/kg) and Diane-35 (1 mg/kg) were given to the treatment subgroups. For comparison, clinical symptoms and histological alterations were noted and documented. Correlation analysis besides Simple Linear Regression among co-variant data was performed. Results: Hair loss, appetite loss, testicular enlargement, depression and elevated body temperature were significant clinical symptoms seen in all treated birds (p<0.01). One and a half percent of the birds who received cyproterone acetate or human chorionic gonadotropin died. According to histological investigation, the heart and skeletal muscle tissues of the majority of treated birds did not change significantly from controls. However, some organ changes, most notably liver failure and testicular darkening, were linked to cyproterone acetate treatment. Conclusion: The results show that both steroids, especially cyproterone acetate, have important physiological effects on common quail. The need for caution while using steroids is highlighted by observed negative effects on vital organs. These investigations also can differentiate between actual and simulated animals using pharmaco-histokinetic markers.