Evaluation of polyphenolic profile, antioxidant, anti-cholinesterase, and anti-alpha-amylase activities of <i>Pistacia lentiscus</i> L. leaves
The phytochemical profile of the hydro-methanolic extract from <i>Pistacia lentiscus</i> L. leaves (MEPLL) was analysed using the LC-ESI-MS/MS method, which identified 17 compounds. The major compounds identified were quinic acid (234.25 mg/g extract), tannic acid (45.92 mg/g extract), quercitrin (2.99 mg/g extract), protocatechuic acid (1.96 mg/g extract), and gallic acid (1.34 mg/g extract). MEPLL exhibited the most potent antioxidant activity, with IC<sub>50</sub> values of 4.79 ± 0.31 µg/mL for DPPH, 2.80 ± 0.02 µg/mL for ABTS, and an A<sub>0.5</sub> value of 3.80 ± 0.64 µg/mL for the phenanthroline assay. Additionally, MEPLL exhibited significant inhibitory effects against acetylcholinesterase (AChE) (IC<sub>50</sub> = 5.76 ± 0.97 µg/mL), butyrylcholinesterase (BChE) (IC<sub>50</sub> = 28.94 ± 1.48 µg/mL), and α-amylase (IC<sub>50</sub> = 8.9 ± 0.14 µg/mL), outperforming the positive controls. Molecular docking studies further supported these <i>in vitro</i> results.
Authors
- Abbas Tarhan (ORCID: https://orcid.org/0000-0001-5196-3892)
- Mustafa Abdullah Yılmaz (ORCID: https://orcid.org/0000-0002-4090-7227)
- Chawki Bensouıcı (ORCID: https://orcid.org/0000-0003-4612-4642)
- Oğuz Çakır (ORCID: https://orcid.org/0000-0002-8006-2054)
- Zahia Kabouche (ORCID: https://orcid.org/0000-0002-3738-6387)
- Saliha Moussaoui
- Assia Guendouze (ORCID: https://orcid.org/0009-0004-3860-0199)
- El Hassen Mokrani (ORCID: https://orcid.org/0000-0002-2725-2940)
- Amina Laribi
- Nesrine Bradai
Institutions
- Dicle University (TR)
- University Frères Mentouri Constantine 1 (DZ)
Publication Details
- Journal
- Natural Product Research
- Published
- 2025-02-24
- DOI
- https://doi.org/10.1080/14786419.2025.2471836
- Citations
- 6
- Primary Topic
- Plant biochemistry and biosynthesis
- Type
- article
- Field-Weighted Citation Impact
- 4.84