Bioactivity profiling of <i>Sanghuangporus lonicerinus</i> : antioxidant, hypoglycaemic, and anticancer potential via <i>in-vitro</i> and <i>in-silico</i> approaches
This study investigates the mycochemical profile and biological activities of hydroethanolic (EtOH), chloroform (CHCl<sub>3</sub>), and hot water (H<sub>2</sub>O) extracts of <i>Sanghuangporus lonicerinus</i> from Uzbekistan. Antioxidant capacity was assessed using 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), NO, and FRAP assays, and <i>in vitro</i> hypoglycaemic effects were evaluated through α-amylase and α-glucosidase inhibition. Antiproliferative potential was explored by analysing the binding affinities of EtOH and H<sub>2</sub>O extracts to estrogen receptor α (ERα), ERβ, androgen receptor (AR), and glucocorticoid receptor (GR), with molecular docking providing structural insights. LC-MS/MS analysis revealed solvent-dependent phenolic profiles, with the EtOH extract containing the highest total phenolic content (143.15 ± 6.70 mg GAE/g d.w.) and the best antioxidant capacity. The EtOH extract showed significant hypoglycaemic effects, with 85.29 ± 5.58% inhibition of α-glucosidase and 41.21 ± 0.79% inhibition of α-amylase. Moderate ERβ binding suggests potential for estrogen-mediated cancer therapy, while strong AKR1C3 inhibition by the EtOH extract supports its therapeutic potential.
Authors
- Nemanja Živanović (ORCID: https://orcid.org/0000-0001-7839-2815)
- Edward T. Petri (ORCID: https://orcid.org/0000-0002-3650-1523)
- Arshad Mehmood Abbasi (ORCID: https://orcid.org/0000-0001-9978-3866)
- Bekhzod Abdullaev (ORCID: https://orcid.org/0000-0001-7798-7382)
- Yusufjon Gafforov (ORCID: https://orcid.org/0000-0003-3076-4709)
- Jovana Mišković (ORCID: https://orcid.org/0000-0002-9717-9259)
- Sofija S. Bekić (ORCID: https://orcid.org/0000-0002-9020-698X)
- Wan Abd Al Qadr Imad Wan‐Mohtar (ORCID: https://orcid.org/0000-0002-5384-4325)
- Young Woon Lim (ORCID: https://orcid.org/0000-0003-2864-3449)
- Manzura Yarasheva (ORCID: https://orcid.org/0009-0006-9195-7917)
- Soumya Ghosh (ORCID: https://orcid.org/0000-0002-9429-5238)
- Milena Jovo Rašeta (ORCID: https://orcid.org/0000-0002-4020-4185)
- Sylvie Rapior (ORCID: https://orcid.org/0000-0002-9105-1561)
- Ikram Abdullaev (ORCID: https://orcid.org/0000-0002-0272-1480)
- Jiajia Chen (ORCID: https://orcid.org/0000-0003-1044-7079)
Institutions
- Centre National de la Recherche Scientifique (FR)
- Academy of Sciences Republic of Uzbekistan (UZ)
- Seoul National University (KR)
- École Pratique des Hautes Études (FR)
- COMSATS University Islamabad (PK)
- University of Novi Sad (RS)
- Université de Montpellier (FR)
- University of Nizwa (OM)
- Abbottabad University of Science and Technology (PK)
- University of Malaya (MY)
- Centre d'Écologie Fonctionnelle et Évolutive (FR)
- Institut de Recherche pour le Développement (FR)
- New Uzbekistan University (UZ)
- National University of Uzbekistan (UZ)
Publication Details
- Journal
- Journal of Enzyme Inhibition and Medicinal Chemistry
- Published
- 2025-02-24
- DOI
- https://doi.org/10.1080/14756366.2025.2461185
- Citations
- 7
- Primary Topic
- Fungal Biology and Applications
- Type
- article
- Field-Weighted Citation Impact
- 9.76