Neuroprotective Effect of Polybion Against Radiation and Cadmium Induced Biochemical Changes in Mice.

The adult healthy Swiss albino mice were procured and maintained at animal house of Govt. Dungar college Bikaner (registration no.1066/go/re/s/07/CPSEA) and provided balanced mice feed and water ad libitum. As to investigate protective effect of Polybion in mice brain against gamma radiation and cadmium chloride alone and in combination also, the animals were divided into seven groups, (control and experimental drug treated and non drug treated).Group I To IV were treated with doses of radiation and Cadmium chloride and group V to VII were treated with . Polybion besides the doses of radiation and Cadmium, seven days prior to irradiation. A minimum of five animals from each group were sacrificed by cervical dislocation and autopsied at each post treatment intervals of 1,2,4,7,14,28 days. Brain is taken out and was kept at -200c.The biochemical estimation of Phospholipids, Total protein, Glycogen ,Cholesterol, Acid Phosphatase activity ,Alkaline phosphatase activity ,DNA and RNA was done in laboratory. All parameters showed an increasing or decreasing trend up to 14-day in non drug treated group and up to 7 days in drug treated groups thereafter all parameters showed signs of recovery after 28 days in non-drug treated group and after 14 days in drug treated group. Early and fast recovery in Polybion treated groups were noticed, Hence Polybion could be used as potent radioprotector during oxidative stress and protect brain from neurological disorders.