Green synthesis of plant extract supported silver nanoparticles using Citrus limonum peel, leaf and seed extract and their antibacterial activity

The synthesis of silver oxide nanoparticles (AgNPs) through the use of plant extracts is a remarkably simple, cost-effective, efficient, and environmentally friendly approach.In recent years, there has been a surge in the exploration of eco-friendly methods for synthesizing AgNPs, with researchers addressing the potential of extracts derived from various plant components, including leaves, stems, roots, and fruits.The current work concentrated on the green synthesis of silver nanoparticles (AgNPs) through the use of aqueous Citrus limonum bark, leaf and seed extract, optimizing the different experimental factors required for the formation and stability of AgNPs.FTIR spectra confirmed that Citrus limonum bark; leaf and seed extract acted as both reducing and surface passivation agent for the synthesized AgNPs.The morphology, size, and elemental composition of AgNPs were investigated by SEM analysis, which showed crystalline spherical silver nanoparticle by XRD analysis.In addition, the antimicrobial and antioxidant properties of this bioactive silver nanoparticle were also investigated.The AgNPs showed excellent antibacterial activity against one (Grampositive) pathogenic bacteria Bacillus cereus and (Gram-negative) Pseudomonas aeruginosa.These results indicated a simple, fast, and inexpensive synthesis of silver nanoparticles using the Citrus limonum bark, leaf and seed extract that has promising antibacterial activity.