Two undescribed benzofuran derivatives from <i>chaetomium elatum</i>

Two undescribed benzofuran derivatives (<b>1</b>-<b>2</b>) were isolated from <i>Chaetomium elatum</i> using silica gel, Sephadex LH-20 column chromatography, and preparative high-performance liquid chromatography (HPLC). The structures of compound <b>1-2</b> were characterised by HRESIMS, NMR, HMBC, NOESY spectral analyses, and ECD calculations. Compounds <b>1-2</b> demonstrated the ability to mitigate the inflammatory response triggered by lipopolysaccharide (LPS) in RAW264.7 macrophages, as evidenced by RT-PCR assay results showing suppression of key inflammatory factors at the concentration of 30 μM. These findings suggest that <b>1-2</b> possess promising anti-inflammatory properties.