<i>Trachyspermum ammi</i> seed extract modulated nanosilver as a transformative agent for antibacterial and wound healing activities

Nanoparticles have gained immense interest as drug-delivery agents. Since the last two decades, they have emerged as probable drugs themselves or may act in symbiotic relation with the drug-loaded. Therefore, this study’s focus is to investigate the efficacy of biosynthesized silver nanoparticles (AgNPs) as antibacterial, antibiofilm, and wound healing agents in in vivo mice models. The AgNPs for this study were synthesized using Trachyspermum ammi seed extract. The characterization of biosynthesized AgNPs, i.e., Trachyspermum ammi silver nanoparticles (TA-AgNPs) was initially done by UV–visible spectroscopy. Further, optimizations of the biophysical characteristics of TA-AgNPs were done by dynamic light scattering, scanning electron microscopy, zeta potential, and Fourier transform infrared spectroscopy. The antibacterial activity of TA-AgNPs was studied against both Gram [Formula: see text] and Gram [Formula: see text] bacteria. The inhibitory effect of AgNPs on biofilm formation was also observed in Bacillus cereus and Escherichia coli. These nanoparticles were then explored for potential in vivo wound healing activity in the Swiss Albino mice model. The results obtained not only showed these green metallic nanoparticles as a potent antibacterial agent that could inhibit biofilm formation but these particles were also able to treat deep wounds without any scar marks. Thus, the biosynthesized AgNPs may be harnessed as remarkable new antimicrobial moieties with wider biomedical applications.