Recycling slaughterhouse waste rumen fluid for biodegradable bioplastic production from <i>Bacillus tequilensis</i> KU 844284

In the present study, bioplastic - producing <i>Bacillus tequilensis</i>, a gram-positive rod, was isolated from slaughterhouse waste rumen fluid. Polyhydroxy butyrate (PHB) was produced from <i>B. tequilensis</i> using nitrogen-deficient Minimal medium (NDMM) and slaughterhouse waste rumen fluid (SHWRF). <i>B. tequilensis</i> produced PHB 4.51 ± 0.06 g/L and 1.60 ± 0.04 g/L from SHWRF and NDMM medium, respectively. Thermal stability, biodegradability, and biocompatibility were among the desirable properties of the PHB that were confirmed by FTIR, XRD, TGA, and DSC analysis. The PHB produced by using the SHWRF medium was degraded at a rate of 60.30% in 45 days. The in vitro cytotoxicity assay using the 3T3-L1 fibroblast cell line demonstrated that it is not harmful. This is the first report of bioplastic production by <i>B.tequilensis</i> using slaughterhouse waste rumen fluid. Rumen fluid from slaughterhouse waste can be recycled to produce biodegradable bioplastic.