Influence of Substrate Composition on Growth, Yield and Antimicrobial Activity of Pleurotus djamor

Aims: This study investigates the cultivation and biological efficacy of Pleurotus djamor var. roseus, commonly known as the pink oyster mushroom, using low-cost techniques and various agro-wastes as substrates. With global mushroom production exceeding two million tons annually, the economic and nutritional importance of mushrooms has been recognized, particularly due to their high protein, fiber content and antioxidant properties. Study Design and Methodology: The research involved preparing a suitable substrates, including the sterilization and spawning of paddy straw, followed by monitoring growth parameters such as spawn running, pinhead formation, and fruit body development. Place and Duration of Study: The study was conducted at Department of Microbiology, Kamarajar Government Arts College, Surandai. Results: The results indicate optimal conditions for mycelial growth and yield, with a biological efficiency of 43% observed in paddy straw substrates. Additionally, the ethanolic extracts of P. djamor were tested for antibacterial activity against both gram-positive and gram-negative pathogens, showing significant inhibitory effects, particularly against Escherichia coli and Pseudomonas aeruginosa. Conclusion: The results indicate that *P. djamor* var. *roseus* is a nutrient-dense mushroom with potential health benefits, making it a valuable addition to diets aimed at addressing chronic diseases and aging. This research underscores the benefits of using agricultural waste for sustainable mushroom cultivation while highlighting the health-enhancing properties of this species.