Determination of ibuprofen by high performance liquid chromatography

Ibuprofen in its pure form and in pharmaceutical formulations (tablets and suspension-dose forms) was determined by high-performance liquid chromatography (HPLC). The analysis was performed on a C18 column (3.9 mm × 150 mm, 5 μm) with a mobile phase consisting of methanol-water-phosphoric acid in ratios [75:24.7:0.3] respectively, and a flow rate of 0.8 mL/min. A UV detector with a wavelength of 214 nm was used. The results were linear in the range of 3,125-100,000 μg/mL for ibuprofen. Correlation coefficients were R = 0.999993 The relative standard deviations (n = 6) for the daytime assay and the inter-day assay were 0.152%, 0.382% This method was characterized by high precision and accuracy and is suitable for quality control in the pharmaceutical industries.