Rapid and high-throughput screening of proteolysis targeting chimeras using a dual-reporter system expressing fluorescence protein and luciferase
The dual-reporter system using both fluorescence and chemiluminescence was successfully constructed. Using this method, we identified effective candidate PROTACs against SIRT2. The dual-reporter system may accelerate drug discovery during PROTAC development.
Authors
- Bole Li
- Yuan Meng (ORCID: https://orcid.org/0000-0002-5409-5610)
- Xuena Yang (ORCID: https://orcid.org/0000-0001-7905-6901)
- Lin Zhang (ORCID: https://orcid.org/0000-0002-8132-7755)
- Jie Zhang
- Xiubao Ren (ORCID: https://orcid.org/0000-0003-4137-2049)
- Wenwen Yu (ORCID: https://orcid.org/0000-0002-7659-068X)
- Shuai Meng (ORCID: https://orcid.org/0009-0001-0052-6008)
- Tianjun Liu (ORCID: https://orcid.org/0000-0002-4661-4417)
Institutions
- Chinese Academy of Medical Sciences & Peking Union Medical College (CN)
- Tianjin Medical University Cancer Institute and Hospital (CN)
- Tianjin haihe hospital (CN)
Publication Details
- Journal
- BMC Biology
- Published
- 2025-02-21
- DOI
- https://doi.org/10.1186/s12915-025-02153-7
- Citations
- 2
- Primary Topic
- Protein Degradation and Inhibitors
- Type
- article
- Field-Weighted Citation Impact
- 1.61