Gain of 20q11.21 in human pluripotent stem cells enhances differentiation to retinal pigment epithelium
We demonstrated that gain of 20q11.21 enhances the speed and yield of RPE differentiation without compromising the phenotype of the derivatives. Finally, we discovered that 20q11.21-localised BCL-XL is important for RPE differentiation with potential non-canonical roles in retinal biology.
Authors
- Zoë Hewitt (ORCID: https://orcid.org/0000-0001-7519-7029)
- Owen Laing (ORCID: https://orcid.org/0000-0003-3862-2529)
- Nidaa A. Ababneh (ORCID: https://orcid.org/0000-0002-2155-3013)
- Fabiha Anjum
- Ivana Barbaric (ORCID: https://orcid.org/0000-0003-3120-9563)
- Peter Coffey (ORCID: https://orcid.org/0000-0002-5427-2939)
- Loriana Vitillo (ORCID: https://orcid.org/0000-0002-7184-1793)
- Duncan Baker (ORCID: https://orcid.org/0000-0002-6475-8246)
Institutions
- University of Jordan (JO)
- Moorfields Eye Hospital NHS Foundation Trust (GB)
- University of California, Santa Barbara (US)
- Regenerative Medicine Institute (MX)
- Sheffield Children's Hospital (GB)
- University College London (GB)
- University of Sheffield (GB)
Publication Details
- Journal
- Stem Cell Research & Therapy
- Published
- 2025-02-21
- DOI
- https://doi.org/10.1186/s13287-025-04196-7
- Primary Topic
- CRISPR and Genetic Engineering
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Lincy Foundation
- California Institute of Regenerative Medicine
- University of Jordan
- King Abdullah II Design and Development Bureau
- UK Regenerative Medicine Platform
- Medical Research Council
- Deanship of Scientific Research, University of Jordan