Biomimetic Recognition of SARS‐CoV‐2 Receptor‐Binding Domain N‐Glycans by an Antiviral Synthetic Receptor
Recognition of glycans by simple synthetic receptors is a key issue in supramolecular chemistry, endowed with relevant implications in glycobiology and medicine. In this context, glycoproteins featuring N-glycans represent an important biological target, because they are often exploited by enveloped viruses in adhesion and infection processes. However, a direct evidence for their recognition by a synthetic receptor targeting N-glycans is still missing in the literature. Using a combination of glycoengineering and mass spectrometry techniques, we present here the direct evidence of biomimetic recognition of complex-type N-glycans exposed on the receptor-binding domain (RBD) of the wild-type spike protein of SARS-CoV-2 by a biologically active, synthetic receptor.
Authors
- Rita Grandori (ORCID: https://orcid.org/0000-0002-6683-9971)
- Renata Grifantini (ORCID: https://orcid.org/0000-0003-0024-3355)
- Raffaele De Francesco (ORCID: https://orcid.org/0000-0001-8754-5123)
- Stefano Roelens (ORCID: https://orcid.org/0000-0002-9763-3263)
- Lorena Donnici (ORCID: https://orcid.org/0000-0002-9153-9102)
- Cristina Nativi (ORCID: https://orcid.org/0000-0002-6312-3230)
- Oscar Francesconi (ORCID: https://orcid.org/0000-0002-6155-4926)
- Mirco Toccafondi
- Francesco Milanesi (ORCID: https://orcid.org/0000-0002-1981-5811)
- Marco Fragai (ORCID: https://orcid.org/0000-0002-8440-1690)
- Carlo Santambrogio (ORCID: https://orcid.org/0000-0003-3986-6971)
- Erika Ponzini (ORCID: https://orcid.org/0000-0002-3103-740X)
- Elisa Pesce (ORCID: https://orcid.org/0000-0003-4214-1904)
Institutions
- Forschungszentrum Jülich (DE)
- University of Milan (IT)
- National Interuniversity Consortium of Materials Science and Technology (IT)
- Istituto Nazionale Genetica Molecolare (IT)
- University of Florence (IT)
- University of Milano-Bicocca (IT)
Publication Details
- Journal
- ChemBioChem
- Published
- 2025-02-21
- DOI
- https://doi.org/10.1002/cbic.202500106
- Citations
- 1
- Primary Topic
- Glycosylation and Glycoproteins Research
- Type
- article
- Field-Weighted Citation Impact
- 0.81
Funders
- Università degli Studi di Milano
- Dipartimenti di Eccellenza
- Università degli Studi di Firenze
- Ministero dell'Università e della Ricerca