Fraction collection of bioactive compounds from ion chromatography: No longer mission impossible

This paper demonstrates the fraction collection of the novel sugar trehalulose, using a modified ion chromatograph. The Ion Chromatography (IC) method, previously published for the analysis of trehalulose, was augmented with a suppressor and purpose-made switching valve unit. A sample of stingless bee honey was then run, following the three main steps:•Separation of trehalulose fraction•Lyophilization•Confirmation of trehalulose The method should be applicable to not only sugar analysis but to any bioactive compound separable by IC. The authors were not able to find a similar method within currently published literature.

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Publication Details

Journal
MethodsX
Published
2025-02-21
DOI
https://doi.org/10.1016/j.mex.2025.103243
Citations
2
Primary Topic
Analytical Chemistry and Chromatography
Type
article
Field-Weighted Citation Impact
1.51
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article

Fraction collection of bioactive compounds from ion chromatography: No longer mission impossible

V. Santiago, Mary T. Fletcher, Hans S. A. Yates, Natasha L. Hungerford et al.
2 citations
MethodsX
Analytical Chemistry and Chromatography
1.51
article

Fraction collection of bioactive compounds from ion chromatography: No longer mission impossible

V. Santiago, Mary T. Fletcher, Hans S. A. Yates, Natasha L. Hungerford, James F. Carter, Ondrea Thompson
article en
2 citations

Abstract

This paper demonstrates the fraction collection of the novel sugar trehalulose, using a modified ion chromatograph. The Ion Chromatography (IC) method, previously published for the analysis of trehalulose, was augmented with a suppressor and purpose-made switching valve unit. A sample of stingless bee honey was then run, following the three main steps:•Separation of trehalulose fraction•Lyophilization•Confirmation of trehalulose The method should be applicable to not only sugar analysis but to any bioactive compound separable by IC. The authors were not able to find a similar method within currently published literature.

MethodsXVol. 14
Queensland Health (AU), The University of Queensland (AU), Thermo Fisher Scientific (United States) (US), Animal, Food and Health Sciences (AU), Thermo Fisher Scientific (Israel) (IL)
Openalex Percentile: Top 21%
Analytical Chemistry and Chromatography
1.51
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Fraction collection of bioactive compounds from ion chromatography: No longer mission impossible — V. Santiago, Mary T. Fletcher, et al. · MethodsX (2025) | TGRS Research Map | TGRS