Immunophenotyping and real-time PCR in clinical treatement of RA patients

Each RA patient has their own unique gene expression and response to therapy.Therefore, their clinical management requires a strictly personalized approach.We used flow cytometry and real-time PCR, together with all other clinical findings, for timely determination of their individual specific biomarkers of disease activity and therapy efficacy.The serum and whole blood specimens, taken from clinically selected RA patients, under corresponding DMARD therapy, were analyzed by BD FACS-CANTO-II four color flow cytometer, for purpose of the therapy effectiveness and Immunophenotyping