Structural changes and cellulose ultrastructure mapped with electron microscopy and SAXS after enzymatic hydrolysis of mildly steam pretreated Norway spruce

The present study reports the microstructural changes caused by pretreatment and enzymatic hydrolysis of Norway spruce. By enzymatically increasing the hemicellulose hydrolysis, the exposed cellulose surface area increases meaning that the cellulose might be easier to access for the enzymes. Structural analysis of biomass after enzymatic hydrolysis can direct the choice of enzymes for improved saccharification efficiency.

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Publication Details

Journal
Biotechnology for Biofuels and Bioproducts
Published
2025-02-21
DOI
https://doi.org/10.1186/s13068-025-02616-7
Primary Topic
Biofuel production and bioconversion
Type
article
Field-Weighted Citation Impact
0.00

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article

Structural changes and cellulose ultrastructure mapped with electron microscopy and SAXS after enzymatic hydrolysis of mildly steam pretreated Norway spruce

Lisbeth Olsson, Eva Olsson, Fabio Caputo, Polina Naidjonoka et al.
Biotechnology for Biofuels and Bioproducts
Biofuel production and bioconversion
article

Structural changes and cellulose ultrastructure mapped with electron microscopy and SAXS after enzymatic hydrolysis of mildly steam pretreated Norway spruce

Lisbeth Olsson, Eva Olsson, Fabio Caputo, Polina Naidjonoka, Maria E. F. Brollo
article en

Abstract

The present study reports the microstructural changes caused by pretreatment and enzymatic hydrolysis of Norway spruce. By enzymatically increasing the hemicellulose hydrolysis, the exposed cellulose surface area increases meaning that the cellulose might be easier to access for the enzymes. Structural analysis of biomass after enzymatic hydrolysis can direct the choice of enzymes for improved saccharification efficiency.

Biotechnology for Biofuels and BioproductsVol. 18(1)
Wallenberg Wood Science Center (SE), Chalmers University of Technology (SE)
Chalmers Tekniska Högskola, Energimyndigheten, Wallenberg Wood Science Center
Clean water and sanitation
Openalex Percentile: Top 97%
Biofuel production and bioconversion
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