Therapeutic Mechanism of Human Platelet Lysate on Spinal Cord Injury in Rats

Objective To screen and systematically analyze the differential proteins in rats suffer from SCI after treating with HPL and thus explore the therapeutic mechanism of HPL on SCI rats.Method 30 SD rats were selected and randomly divided into three groups: control group, injury group, and treatment group.Allen Test was performed to establish the rat models with injured spinal cord, and 2 mL of HPL was intraperitoneally injected into rats in the treatment group every day.After 14 consecutive days of injection, the thoracic spine of rats was sampled and analyzed by proteomics.Result A total of 61 differential proteins were screened in the injury and treatment groups, including 7 upregulated proteins and 14 down-regulated proteins.The GO enrichment analysis results showed that the differential proteins included cellular components such as ATP dependent chromatin remodeling complex composed of multiple subunits, recycling endosome, extracellular space, and ATP synthase complex.These differential proteins promote serine-type endopeptidase inhibitor activity, myosin-binding protein and peptidase inhibitor molecular functions, and are involved in biological processes including fibrinolysis, sulfur component synthesis and metabolism, cellular modification of amino acid metabolism, and myelination.The KEGG analysis results mainly suggested the therapeutic mechanism of HPL for SCI through nitrogen metabolism, sodium taurocholate hydrate, complement coagulation cascade, and alcoholic liver disease.Conclusion HPL has a good therapeutic effect on SCI via the bioinformatic mechanisms above.