Ipomoea aquatica derived Synthesis of Silver Nanoparticles to Control Multiple Antibiotic-Resistant Microorganisms Isolated from Medical Wastage

The green synthesis of silver nanoparticles (AgNPs) exhibit potent antibacterial, antifungal, antiviral, anticancer and antioxidant properties. The goal of this study is to synthesis and characterization of AgNPs using leaf extract from Ipomoea aquatica and antimicrobial (Bacteria, Fungi) activity that are resistant to antibiotics that were found in medical waste from Kushtia Sadar Hospital, such as disposable syringes and needles. The synthesis of silver nanoparticles was primarily confirmed by the color change (brown) of the sample solution. Additionally, characterization was performed via UV-vis spectroscopy, Surface Plasmon Resonance (SPR) peaks showed up at 454 nm with an absorbance of 1.287 for 1:2, 461 nm with an absorbance of 1.322 for 1:4, 452 nm with an absorbance of 1.632 for 1:6, 466 nm with an absorbance of 0.943 for 1:8, 446 nm with an absorbance of 0.699 for 1:10, 448 nm with an absorbance of 0.622 for 1:12 ratios. Multiple antibiotic resistance bacteria were isolated from the medical wastages which were controlled effectively by applying synthesized nanoparticles at different doses. Bacterial Strain BSN7 is the most sensitive strain, having a 13±0.208 mm inhibitory zone at a concentration of 60μg/mL of AgNPs. BSN8 and BSN9 are the most sensitive at 60μg/mL concentration of AgNPs with inhibitory zones of 11±0.2 mm and 12±0.153 mm respectively. Whereas at the concentration of 15μg/mL the zone of inhibition for bacterial strains BSN7, BSN8 and BSN9 are 6±0.1, 7±0 and 8±0.058 which is considered as MIC (Minimal Inhibitory Concentration). Eventually, it can be stated that synthesized silver may play a significant role in developing a novel therapeutic strategy to manage infections that are resistant to antibiotics. After antibacterial investigation, AgNPs were subsequently used for the assessment of antifungal efficacy.