Immunomagnetic enrichment coupled to PAX8/TP53 molecular pathology approach increases sensitivity in the detection of ovarian cancer cells in ascites
High-grade serous ovarian carcinoma (HGSOC) is one of the deadliest malignancies in female population and the cause of 70% of all ovarian cancer-related deaths. Among its hallmarks, the fluid accumulation in the peritoneal cavity, or ascites, is a peculiar pathological sign during late stages and in recurrent patients. Besides cancer cells, ascitic fluids contain a heterogeneous cellular composition, representing a precious source to dissect molecular mechanisms underlying invasion and metastatization or find new biomarkers to predict therapy response. However, malignant cells are often a minority population in ascites making the detection and analysis of cancer cells a challenge. Here we propose a combinatorial approach for the detection of malignant cells in OC ascites based on <i>TP53</i> deep sequencing and PAX8 cytological staining. In addition, we improve the procedure by implementing a cancer cell enrichment step, increasing the sensitivity in the detection of neoplastic fraction and potentiating downstream research and diagnostics applications.
Authors
- Giuseppe Gasparre (ORCID: https://orcid.org/0000-0003-1229-9006)
- A Ricciardi (ORCID: https://orcid.org/0000-0002-0584-0194)
- Manuela Sollazzo (ORCID: https://orcid.org/0000-0001-5451-0063)
- Anna Myriam Perrone (ORCID: https://orcid.org/0000-0003-3140-4772)
- Stella Di Costanzo (ORCID: https://orcid.org/0000-0003-2877-0261)
- Sara Milioni
- Anna Maria Porcelli (ORCID: https://orcid.org/0000-0002-6209-0692)
- Sara Coluccelli (ORCID: https://orcid.org/0000-0002-1475-4044)
- Dario de Biase (ORCID: https://orcid.org/0000-0002-0609-8817)
- Stefano Miglietta (ORCID: https://orcid.org/0000-0002-7755-2028)
- Monica De Luise (ORCID: https://orcid.org/0000-0002-3486-3985)
- Ivana Kurelac (ORCID: https://orcid.org/0000-0002-8364-9985)
- Thais Maloberti (ORCID: https://orcid.org/0000-0002-8306-4653)
- Luisa Iommarini (ORCID: https://orcid.org/0000-0002-6804-7302)
- Marco Grillini (ORCID: https://orcid.org/0000-0003-2506-7239)
- Elena Luppi (ORCID: https://orcid.org/0000-0001-5488-2430)
- Camelia Alexandra Coadǎ (ORCID: https://orcid.org/0000-0001-8362-6639)
- Beatrice Cavina (ORCID: https://orcid.org/0000-0001-5485-5360)
- Moira Ragazzi (ORCID: https://orcid.org/0000-0002-8761-9265)
- Pierandrea De Iaco (ORCID: https://orcid.org/0000-0002-8841-6531)
- Francesca Nanetti
- Luigi D’Angelo (ORCID: https://orcid.org/0000-0002-0043-7371)
- Laura Lanteri
- Samantha Corrà (ORCID: https://orcid.org/0000-0003-1985-6194)
Institutions
- University of Modena and Reggio Emilia (IT)
- Azienda Sanitaria Unità Locale di Reggio Emilia (IT)
- Azienda USL di Bologna (IT)
- Istituti di Ricovero e Cura a Carattere Scientifico (IT)
- University of Bologna (IT)
Publication Details
- Journal
- Frontiers in Molecular Biosciences
- Published
- 2025-02-20
- DOI
- https://doi.org/10.3389/fmolb.2025.1537407
- Citations
- 2
- Primary Topic
- Cancer Genomics and Diagnostics
- Type
- article
- Field-Weighted Citation Impact
- 1.87