The Regenerative Potential of Rice Bran Oil Incorporated into Nanofibers: An In Vitro Study

Background/Objectives: Rice bran oil (RBO) is rich in phytochemical compounds and has many pharmaceutical applications. This work evaluated the regenerative potential of nanofibers incorporating RBO, focusing on their efficacy in tissue engineering and dermatological formulations. The main objective was to investigate the impact of RBO on SK-MEL-28 melanoma cell migration and wound closure through an in vitro healing assay. In addition, the biocompatibility and cell adhesion properties of the nanofibers were examined. Methods: The study employed cell culture techniques and field emission gun scanning electron microscopy (FEG-SEM) investigation. RBO was tested at different concentrations (0.5%, 1%, 5%, or 10%), both in isolation and incorporated into nanofibers. Cell migration was assessed through a wound-healing assay, while cell adhesion to the nanofibers was assessed using FEG-SEM. Statistical analyses were conducted to assess the significance of the findings. Results: Higher cell migration was achieved with 5% (p < 0.002) and 10% (p < 0.05) RBO nanofibers compared to the control and isolated RBO. The biocompatibility study found cell adhesion capability, highlighting the potential of these nanofibers for tissue engineering applications. Conclusions: Our results showed enhanced SK-MEL-28 cell migration and wound closure with RBO-incorporated nanofibers compared to isolated RBO. Biocompatibility was confirmed, suggesting potential for tissue engineering. Our findings indicate that the incorporation of RBO into nanofibers improves their oxidative stability, which is essential for preserving their phytochemical compounds and their beneficial effects on human skin cells in vitro.