Feeder-cell-free system for ex vivo production of natural killer cells from cord blood hematopoietic stem and progenitor cells
Our ex vivo culture process supports scalable ProT-NK cell production in high yields, reducing dependency on feeder cells and mitigating contamination risks. Our findings demonstrate the feasibility of generating large, functional NK cell populations from HSPCs isolated from readily available cord blood sources and offer an efficient alternative to PB-NK cell therapies.
Authors
- Tifanie Blein (ORCID: https://orcid.org/0000-0002-3416-3786)
- Chantal Lagresle‐Peyrou (ORCID: https://orcid.org/0000-0002-2216-6453)
- Pauline Rault
- Pierre Gaudeaux (ORCID: https://orcid.org/0000-0001-9179-9775)
- Hanem Sadek (ORCID: https://orcid.org/0000-0003-0756-0193)
- Tayebeh-Shabi Soheili
- Olivier Nègre
- Ranjita Devi Moirangthem (ORCID: https://orcid.org/0000-0003-2182-0959)
- Juliette Paillet (ORCID: https://orcid.org/0000-0001-7118-7165)
- Isabelle André‐Schmutz (ORCID: https://orcid.org/0000-0002-3905-9910)
- Asma Berriche
- Marta Martin Corredera
- Jeanne Roche-Naude
Institutions
- Inserm (FR)
- Université Paris Cité (FR)
- Assistance Publique – Hôpitaux de Paris (FR)
- Institut des Maladies Génétiques Imagine (FR)
Publication Details
- Journal
- Frontiers in Immunology
- Published
- 2025-02-20
- DOI
- https://doi.org/10.3389/fimmu.2025.1531736
- Citations
- 4
- Primary Topic
- Immune Cell Function and Interaction
- Type
- article
- Field-Weighted Citation Impact
- 3.19