PUNCH: An Interactive Web Server for Predicting Intrinsically Disordered Regions in Protein Sequences

PUNCH is a freely accessible web server designed for the rapid and accurate prediction of intrinsically disordered regions (IDRs) in protein sequences. Built on a high-performance computational framework, PUNCH web server which built on PUNCH2-Light predictor, combines speed with predictive accuracy, offering users a streamlined interface for generating predictions from sequence input. Validated against the CAID2 benchmarking datasets, PUNCH web server demonstrates competitive performance in detecting IDRs across diverse protein sequences. Notably, it excels in the Disorder_PDB dataset and provides reliable results for the Disorder_NOX dataset, addressing the challenges of predicting disordered regions with low sequence similarity. The server is available at https://alienlabs.ucd.ie/punch2/, with extensive documentation and downloadable example datasets to support researchers in structural biology and bioinformatics.