Blockade of P2X7 receptors preserves blood retinal barrier integrity by modulating the plasmalemma vesicle‐associated protein: Implications for diabetic retinopathy
High levels of glucose increased PLVAP expression in HRMECs, which was blocked by JNJ47965567. Furthermore, JNJ47965567 preserved VE-cadherin and ZO-1. In the choroidal vasculature of diabetic mice, PLVAP immunostaining was increased, compared to levels in non-diabetic mice. This increase was significantly attenuated by treatment with JNJ47965567 CONCLUSIONS AND IMPLICATIONS: This study showed that P2X7 receptor signalling is an important component of a complex gene regulatory network, including PLVAP, mediating the pathophysiology of diabetic retinopathy. The P2X7 receptor antagonist JNJ47965567 showed a good pharmacodynamic profile, suggesting that this approach could be of value in the treatment of diabetic retinopathy.
Authors
- Kenneth Patrick Mitton (ORCID: https://orcid.org/0000-0002-4751-7440)
- Cornel Baltă (ORCID: https://orcid.org/0000-0001-5734-0071)
- Federica Conti
- Giuseppe Nicosia (ORCID: https://orcid.org/0000-0002-0650-3157)
- Alina Ciceu (ORCID: https://orcid.org/0000-0001-6502-2655)
- Francesca Lazzara (ORCID: https://orcid.org/0000-0002-9825-2104)
- Hildegard Herman (ORCID: https://orcid.org/0000-0002-6741-0245)
- Anca Hermenean (ORCID: https://orcid.org/0000-0001-8510-6653)
- Naomi Haque
- Chiara Bianca Maria Platania (ORCID: https://orcid.org/0000-0002-8630-5993)
- Maria Consiglia Trotta (ORCID: https://orcid.org/0000-0001-9813-5955)
- Michele D’Amico (ORCID: https://orcid.org/0000-0002-6899-0595)
- Claudio Bucolo (ORCID: https://orcid.org/0000-0002-4879-4140)
- Settimio Rossi (ORCID: https://orcid.org/0000-0002-8682-6860)
- Filippo Drago
- Wendelin Dailey
- Erika Giuffrida
Institutions
- Oakland University (US)
- University of Campania "Luigi Vanvitelli" (IT)
- University of Catania (IT)
- Vasile Goldis Western University of Arad (RO)
- Smith-Kettlewell Eye Research Institute (US)
Publication Details
- Journal
- British Journal of Pharmacology
- Published
- 2025-02-20
- DOI
- https://doi.org/10.1111/bph.70007
- Citations
- 4
- Primary Topic
- Adenosine and Purinergic Signaling
- Type
- article
- Field-Weighted Citation Impact
- 4.47
Funders
- Ministero dell’Istruzione, dell’Università e della Ricerca
- National Institutes of Health
- National Eye Institute
- Precursory Research for Embryonic Science and Technology