Effect of different forms of lysine and methionine in broiler diets on the key genes transcriptional profile

The. aim of the. study was to evaluate the effect of compound feeds with reduced (by 5%) nutritional content of lysine, methionine and metabolic energy when lysine and methionine are included in them in various forms during their interaction with broiler sex on the transcription profile of key genes regulating antioxidant protection, the immune system, inflammation and apoptosis, productivity and barrier function of the gastrointestinal epithelium. Methods. The experiment was carried out at the “Zagorskoye” in 2024 on a poultry meat of the “Smena 9” cross from 1 to 35 days of age. Results. The live weight of rooster and hens in experimental groups II and IV was slightly higher compared to control I, whereas in group III it was lower. In many cases, changes in the composition of the diet of rooster and hens had a significant impact on the expression of a number of key genes. In roosters, PTGS2 expression in experimental groups II–IV increased sharply (from 4.9 to 52.0 times) compared with control I, whereas in hens it increased only 1.5–2.3 times. The expression of the Muc2 gene mRNA in hens decreased in experimental group II by 1.9 times compared with group I, whereas in roosters, on the contrary, it increased by 3.1 times.