Identification of two glycosyltransferases required for synthesis of membrane glycolipids in <i>Clostridioides difficile</i>

<i>Clostridioides difficile</i> infections are the leading cause of healthcare-associated diarrhea. <i>C. difficile</i> poses a risk to public health due to its ability to form spores and cause recurrent infections. Glycolipids make up ~50% of the polar lipids in the <i>C. difficile</i> membrane, a higher percentage than other common pathogens and include a unique glycolipid not present in other organisms. Here, we identify glycosyltransferases required for the synthesis of glycolipids in <i>C. difficile</i> and demonstrate the important role glycolipids play in <i>C. difficile</i> physiology.