DNA Barcoding for the Assessment of the Taxonomy of Fish from Chilika Lagoon, India

DNA barcoding is a technique in which identification of species using DNA barcodes. We generated a verified reference library of cytochrome c oxidase subunit I (COI) sequences for 226 barcodes belonging to 83 fish species from 67 genera, 39 families and 21 orders of fishes with the average divergence within a species is 010%, 13.57% within a genus, and 17.33% within a family with 97-100% identity with comparison to the Genbank database and BOLD of the Chilika lagoon, India. Data analysis done by barcode gap analysis, barcode index number and automatic barcode gap discovery to discriminate the species up to 97.53%, 93.90% and 95.06% respectively. The Barcode Index Number (BIN) discordance analysis reflected 226 specimens belongs to 83 BINs, of which 73.49%were taxonomically concordant and 26.50% were singletons and absence of discordant BIN in our dataset. Finally, the identification success rate of DNA barcoding was evaluated based on the sequencing success rate and species discrimination rate.