Screening and Production of Extracellular Fungal Laccase from Peniophora Albobdia by Solid State Fermentation with Agro-industrial Waste

The enzyme Laccase is known to degrade many phenolic, aromatic compounds. The present study describes, fungal fruiting body collected from Chhatrapati Sambhajinagar, isolated on 2% Malt Extract Agar and substrate oxidation studies carried out to screen fungi for laccase production by using different laccase substrate. The aim of this study was to extract extracellular laccase from Peniophora albobdia with solid state fermentation by different agro industrial wastes like Tur bagasse, Corn stubs, Soybean bagasse, Groundnut bagasse, sugarcane bagasse, and brewer’s spent grain. A utilization of biomass waste had applied in this research. The enzyme activity unit of the different types of substrates was significantly different. The highest activity unit was obtained by Brewers spent grain than the other substrates. From results it is confirmed that the best substrate for laccase production is brewers spent grain with nutrient addition. The statistical optimization needs to be carried to increase the production of laccase enzyme which will help to bio-eliminate the various industrial phenolic waste compounds.