Fabrication of the Plant Extract Loaded Antimicrobial Nanofiber for Biofilm Associated Infections

Abstract This study aims to determine the chemical composition of Stachys tmolea and to assess its antimicrobial potential for biomedical applications. The 53 phytochemicals of Stachys tmolea were elucidated by liquid chromatography–electrospray tandem mass spectrometry. The rich phytochemical profile of Stachys tmolea highlights the prevalence of quinic acid, chlorogenic acid, and cosmosiin in the plant's methanol extract. The electrospinning technique was used to obtain polycaprolactone/chitosan/sericin nanofibers. The nanofiber structure is characterized by SEM, XRD, FITR, and TGA. The highest antimicrobial activity (19 ± 1.00) was found in 100 mg/mL of plant extract against Staphylococcus aureus ATCC 25923 and was evaluated as susceptible. Staphylococcus aureus ATCC 25923 and Listeria monocytogenes ATCC 1911 were found to be sensitive to plant extract loaded nanofiber. Antibiofilm test results revealed that the most effective group was determined as 2 x MIC of the plant extract (72% inhibition) for biofilm inhibition of Staphylococcus aureus ATCC 25923.