Assessment of the quality of surface waters of a drinking reservoir using the method of biotesting on phytoplankton cells

The article discusses the possibilities of using methods for recording light and induction curves of chlorophyll fluorescence of test-culture Chlorella vulgaris in biotesting of water samples. The results of biotesting of water samples from the Volyntsevo reservoir and its main tributaries are presented. The data are presented for the period of active use of reservoir resources, which led to a significant decrease in water levels and deterioration in the quality of water resources. Studies have shown the presence of chronic toxic effects of samples from the reservoir on the test object under study. The filtrate of water samples from the northern tributaries of the Volyntsevo reservoir had a stimulating effect on phytoplankton culture. The methods of fluorimetric analysis confirm the results of the biotesting technique, are consistent with changes in the number of cells and the content of chlorophyll in the samples, and also reveal a negative effect on the photosynthetic apparatus of Chlorella vulgaris, which does not cause changes in the parameters of the growth of the test culture. The negative impact of water samples from the main tributary of the reservoir, the Bulavin River, on the speed of electronic transport and the functioning of primary electron acceptors of photosystem II of Chlorella vulgaris cells was revealed. The obtained materials can be useful for researchers dealing with the problems of biophysics of primary photosynthetic reactions, environmental monitoring of surface natural waters and biotesting.