Photoinduced Actin Aggregation Involves Cell Death: A Mechanism of Cancer Cell Cytotoxicity after Near-Infrared Photoimmunotherapy
Near-infrared photoimmunotherapy (NIR-PIT) is a cancer treatment modality that uses antibody-photoabsorber (IR700) conjugates to destroy specific cells. The reaction between the antibody and photoabsorber is triggered by NIR-light, and this alters the shape and hydrophilicity of the conjugate. This photochemical reaction is responsible for NIR-PIT-induced cell death; however, the detailed mechanism underlying this effect remains unknown. In this study, we demonstrated that actin filaments underneath the cell membrane play an important role in NIR-PIT-induced cell death and that IR700 mediates the photochemical reaction of the conjugates, leading to actin filament aggregation upon NIR-light irradiation. The destruction of cortical actin beneath the cell plasma membrane allows water to flow into the cell based on osmotic conditions, resulting in cell rupture. This sequence of events may constitute the mechanism of NIR-PIT-induced cell death, making NIR-PIT a promising cancer treatment modality.
Authors
- Kazuhide Sato (ORCID: https://orcid.org/0000-0003-3025-088X)
- Hirotoshi Yasui (ORCID: https://orcid.org/0009-0006-6036-2486)
- Rika Fukushima (ORCID: https://orcid.org/0000-0002-8572-1901)
- Toshihiko Ogura (ORCID: https://orcid.org/0000-0001-8953-6562)
- Tomoko Okada
- Misae Shimizu
- Ryu Okada (ORCID: https://orcid.org/0000-0003-4142-446X)
- Yuko Nishinaga
- Mitsuo Sato (ORCID: https://orcid.org/0000-0001-5458-9576)
- Kazuomi Takahashi
- Chiaki Koike
- Ayako Kato (ORCID: https://orcid.org/0009-0002-8785-9186)
- Y Isobe
- Mizuki Yamada
- Shunichi Taki
Institutions
- Japan Science and Technology Agency (JP)
- Centre for Research in Engineering Surface Technology (IE)
- Forest Research (GB)
- Centre de Recherche en Économie et Statistique (FR)
- Center for Responsible Travel (US)
- Imaging Center (US)
- Nagoya University (JP)
- National Institute of Advanced Industrial Science and Technology (JP)
Publication Details
- Journal
- ACS Nano
- Published
- 2025-02-18
- DOI
- https://doi.org/10.1021/acsnano.5c00104
- Citations
- 5
- Primary Topic
- Nanoplatforms for cancer theranostics
- Type
- article
- Field-Weighted Citation Impact
- 3.37
Funders
- Ministry of Education, Culture, Sports, Science and Technology
- Japan Society for the Promotion of Science
- Core Research for Evolutional Science and Technology
- Fusion Oriented REsearch for disruptive Science and Technology